Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 2: 34 - 39. Published February 2008.
Control of Rhizoctonia solani in Soybean (Glycin max L) by seed-coating with Trichoderma viride and Gliocladium virens spores
Nahed Z. Haikal
*Botany Department, Faculty of Science, Cairo University, Giza 12316, Egypt
Address for correspondence: Email: tarekmoussa@yahoo.com; Tel. 0020235676654
ABSTRACT
Objectives: The study evaluated biocontrol of root-rot disease caused by Rhizoctonia solani on soybean using two antagonistic fungal species Trichoderma viride and Gliocladium virens, applied as seed coating or culture filtrate.
Methodology and Results: Pathogenic R. solani was added to sterile vermiculite in pots to give an inoculum density of 1- 5 g fresh weight mycelium/kg of sterile vermiculite. Sterilized soybean seeds were treated before planting by soaking for 30 min in 4 x106 CFU/ ml spore suspensions of either T. viride or G. virens. Coating soybean seeds with T. viride spores reduced the incidence of root-rot disease by up to 83% in the greenhouse experiment. Coating seeds with G. virens spores also decreased disease incidence but to a lesser extent than T. viride. Cell free culture filtrates of both T. viride and G. virens also significantly inhibited the growth of R. solani, with effect increasing as the concentration of the filtrates in the culture media increased. The root exudates of soybean seedlings increased the growth of the pathogenic fungus.
Potential application of findings: The results show that coating soybean seeds with the spore suspension of T. viride or G. virens can effectively control R. solani on soybean.
Key words: biocontrol, Gliocladium, Rhizoctonia, Soybean, Trichoderma,
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