Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 14: 768 - 774. Published February 2, 2009.
Methanolic extract of Indigofera tinctoria induces apoptosis in HCT116 cells
Magesh V.*^, Yuvaraj G.* Deecaraman M.* and Kalaichelvan PT.**
*Dr. MGR Educational and Research Institute, Dept of Biotechnology, Chennai; **University of Madras, CAS in Botany, Chennai.
^Corresponding author e-mail: mahesharavind2006@yahoo.co.in
ABSTRACT
Objective: To determine the anticancer activity of the methanol extract of Indigofera tinctoria, which induces apoptosis in HCT 116 colon cancer cell line.
Methodology and results: the effect of the methanolic extract of Indigofera tinctoria on HCT 116 cells was determined by cell viability, DNA fragmentation and comet assay. Treatment of HCT 116 cell lines with various concentrations of the extracts inhibited growth and induced apoptosis in a dose-dependent manner.
Conclusion and application of results These results indicate that methanol extract of I. tinctoria exhibits antiproliferative effect on HCT 116 cells via apoptosis, and it may be a potential candidate in the field of anticancer drug discovery.
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