Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 - 5902]
Volume 66: 5040- 5048 Published June 30, 2013.
Phytochemical
screening and study of comparative antibacterial activity of aqueous
and alcoholic extracts of the leaves and barks of Terminalia catappa on
multiresistant strains.
Rubens Dinzedi
Mbengui1, 2, Nathalie K. Guessennd 2, Gervais M. M’boh3, Julien K.
Golly1; Constantin O Okou1; Jean D. Nguessan1; Mireille Dosso2 ; Joseph
A Djaman 1,31 Biochemical Pharmcodynamie Laboratory, University Felix Houphouet Boigny 22 BP 582 Abidjan 22, Côte d'Ivoire,
2 Department of Bacteriology and Virology - Institute Pasteur of Côte d'Ivoire, 01 BP 490 Abidjan Côte d'Ivoire
3 Department of clinical and fundamental Biochemistry - Institute Pasteur of Côte d'Ivoire, 01 BP 490 Abidjan, Côte d'Ivoire
Corresponding author: rdinzedi@yahoo.fr ; Tel. No. +225 05621524/ 48306965
Original submitted in on 13th April 2013 Published online at www.m.elewa.org on 30th June 2013.
ABSTRACT
Objectives:
In this study, methanol, ethanol and aqueous extracts of Terminalia
catappa L. leaves and barks were compared for antibacterial activity
against clinical (sensitive and multidrug-resistant) and ATCC strains
of Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus
(MRSA).
Methodology and Results: The confirmation of the Enterobacteriaceae Extended-Spectrum-β- Lactamases (ESBL) producing strains was done by the double-disc synergy tests, the MRSA by disk diffusion method (cefoxitin 30 μg) according to the methods of CA-SFM, 2009. The agar dilution and broth dilution method was used for the determination of the antimicrobial parameters (MIC and MBC) on these sensitive, ESBL producing and MRSA strains. The bark extracts generally showed better activity compared to leaves extracts; but methanolic extracts of barks were the most active of all the extracts, exhibiting a broad-spectrum activity. However, Staphylococcus aureus were sensitive compared to Escherichia coli and Klebsiella pneumoniae. Antibacterial activity showed value ranged from 0.52 ± 0.13 to 4.16 ± 1.04 mg/ml (MIC) and 1.56 to 10.42 ± 2.08 mg/ml (MBC). Phytochemical screening showed the presence of Flavonoids, Alkaloid, Steroid, Terpenoids, Gallic Tannins, Saponins and with the exception of the aqueous extract for Coumarins.
Conclusions: The results obtained suggest that methanolic extracts of T. catappa barks can be used in treating bacterial diseases.
Key words: Phytochemical Screening; Comparative Antibacterial activity; Terminalia catappa; multiresistant strains.Methodology and Results: The confirmation of the Enterobacteriaceae Extended-Spectrum-β- Lactamases (ESBL) producing strains was done by the double-disc synergy tests, the MRSA by disk diffusion method (cefoxitin 30 μg) according to the methods of CA-SFM, 2009. The agar dilution and broth dilution method was used for the determination of the antimicrobial parameters (MIC and MBC) on these sensitive, ESBL producing and MRSA strains. The bark extracts generally showed better activity compared to leaves extracts; but methanolic extracts of barks were the most active of all the extracts, exhibiting a broad-spectrum activity. However, Staphylococcus aureus were sensitive compared to Escherichia coli and Klebsiella pneumoniae. Antibacterial activity showed value ranged from 0.52 ± 0.13 to 4.16 ± 1.04 mg/ml (MIC) and 1.56 to 10.42 ± 2.08 mg/ml (MBC). Phytochemical screening showed the presence of Flavonoids, Alkaloid, Steroid, Terpenoids, Gallic Tannins, Saponins and with the exception of the aqueous extract for Coumarins.
Conclusions: The results obtained suggest that methanolic extracts of T. catappa barks can be used in treating bacterial diseases.
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