Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 17(1): 2514 - 2521. Published February, 2013.
Optimization of extraction process of escin from dried seeds of Aesculus hippocastanum L. by Derringer’s desirability function
Zorica Vujica*, Dragana Novovicb, Ivana Arsicc, Dusanka Anticba University
of Belgrade-Faculty of Pharmacy,
Vojvode Stepe 450, 11 000 Belgrade, Republic of Serbia
bAlternativa
medica, Manufecturer of phytopreparations, Zmaj Jovina 1, 15 300 Loznica,
Republic of Serbia
c University
of Niš-Faculty of Medicine, Department of Pharmacy, Bulevar dr Zorana Djindjica
81, 18 000 Nis, Republic of Serbia
*Corresponding author E-mail address: zvujic@pharmacy.bg.ac.rsTel.: +381-11-39-51-340; fax: +381-11-29-72-840.
SUMMARY
The
optimization of the extraction procedure of escin from dried seeds of
Aesculus hippocastanum L. was studied. Full factorial design with
central point and Derringer’s desirability function was employed to
obtain the best possible combination of temperature (A: 25–45 ◦C), flow
rate of organic solvent (B: 125-345 ml min-1) and extraction time (C:
3–6 h) for maximum extraction yield of escin. The experimental data
were fitted to a second-order polynomial equation using multiple
regression analysis and also analyzed by appropriate statistical
methods (ANOVA). In order to get the best chromatographic performance,
the multicriteria methodology was employed by means of Derringers
desirability function. The optimum extraction conditions were as
follows: the temperature of 45C, flow rate of 125 ml min-1 and
extraction time 3h.
FULL PAPER [PDF AVAILABLE HERE ]
