Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 19(2): 2888 - 2900. Published September, 2013.
Modulation of Expression, Localization of FSHR and Uterine Development by GnRH Agonist Active Immunization in Ewes.
Wei Suocheng1*, Gong Zhuandi2, Ouyang Xiahui1, Xie Kun3, Wei Min1,
1 College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, P. R. China
2 Medicine College, Northwest University for Nationalities, Lanzhou 730030, P. R. China
3 Basic Medicine College, Lanzhou University, Lanzhou 730030, P. R. China
*Corresponding author: Suocheng Wei (E-mail: weisc668@163.com ),
College of Life Science and Engineering, Northwest University for
Nationalities, No. 1, Xibeixincun Chengguan District, Lanzhou, Gansu
Province, 730030, P.R. China.
Key words: Gonadotropin-releasing hormone agonist; FSH receptor;
protein expression; microstructure; immunohistochemistry; uterus; ewe
1 SUMMARY
The present study was designed to investigate the effects of
gonadotropin-releasing hormone (GnRH) agonist immunization on the
expression of follicle stimulating hormone receptor (FSHR) mRNA in the
pituitary and FSHR protein in the uteri, also to confirm the efficacy
on uterine development. 42 ewes, 5 to 6 months of age, were assigned to
6 experimental groups (EG, n=7 per group). Animals in EG-Ⅰ, EG-Ⅱand
EG-Ⅲ were subcutaneously injected with 200 μg, 300 μg and 400 μg
alarelin antigens twice (on day 0, 28 and 14), respectively. Animals in
EG- Ⅳ and EG-Ⅴ were subcutaneously injected with 200 μg and 300 μg
alarelin antigen, four times (on day 0, 7, 14 and 21), respectively.
Animals in the Control Group (CG) were subcutaneously injected with 2.0
mL of GnRH agonist solvent twice (on day 0 and 14). The pituitary and
uterine horns were dissected aseptically on day 70 and weighed
immediately using an electronic balance. The expression of FSHR protein
in the uteri was detected using Western blotting. Fluorescence
quantitative RT-PCR was implemented to measure the expression of FSHR
mRNA in the pituitary. Tissue slices were observed under light and
electron microscopes and photographed. The results showed that alarelin
active immunization can promote the expression of FSHR protein in the
uteri, increase the localization of FSHR in uteri, and inhibit the
expression of FSHR mRNA in the pituitary gland. It can also lessen
endometrial epithelium thickness and uterine wall thickness, decrease
the uterine weights, similarly affect the microstructure and
ultrastructure of uteri, resulting in the inhibition of uterine
development dose-dependently.
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