Bankole’s Abstract JABS Vol. 73

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Journal of Applied Biosciences (J. Appl. Biosci.) [ISSN 1997 – 5902]

Volume 73: 6027 – 6032 Published January 31, 2014.

Identification de Staphylococcus aureus : est-il possible de réduire le volume de plasma de lapin sans influencer la recherche de la staphylocoagulase libre ?

Bankolé1 H.S., Dougnon*1 T.V., Amoussou1 A.N., Fiogbé1 E.P., Baba-Moussa2 L.
1Ecole Polytechnique d’Abomey-Calavi, Laboratoire de Recherche en Biologie Appliquée, Université d’Abomey-Calavi, 01 BP 2009 Cotonou, Bénin.
2Université d’Abomey-Calavi, Faculté des Sciences et Techniques, Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, 05 BP 1604 Cotonou, Bénin
*Auteur pour la correspondance : Dr Dougnon T. Victorien, Tél. 00 229 97 73 64 46, victorien88@hotmail.com

Original submitted in on 16th November 2013 Published online at www.m.elewa.org on 31st January 2014.

RESUME

Objectif : Le coût élevé du plasma lyophilisé de lapin utilisé dans le cadre de l’identification des souches de Staphylococcus aureus constitue une des raisons d’inadéquation entre les dépenses et les recettes des laboratoires d’analyses biomédicales. Pour contourner cette difficulté, les techniciens de laboratoire ont recours à des méthodes d’identification moins coûteuses. La présente étude a eu pour objectif de déterminer dans un but économique le plus petit volume de plasma de lapin capable de révéler la présence de la staphylocoagulase libre.
Méthodologie et résultat : Pour ce faire, 25 souches de Staphylococcus aureus isolées de différents échantillons biologiques et identifiées sur la base de la présence de la staphylocoagulase libre ont été utilisées. Ces souches ont été soumises à la recherche de la staphylocoagulase libre avec des volumes décroissants de 0,4 ml ; 0,3ml ; 0,2ml et 0,1ml de plasma lyophilisé de lapin. Toutes les souches bactériennes ont montré un résultat positif en 24 heures avec 0,2 ml comme plus petit volume. Le coagulum formé présente la même consistance que celui obtenu avec le volume de 0,5 ml indiqué par le protocole normal.
Conclusion et application de résultats: Au total, le volume de plasma de lapin peut-être donc réduit de 0,5 à 0,2 ml sans aucun impact sur la qualité du résultat. Dans le contexte de pays moins avancé comme le Bénin, cela est un premier jet en vue de l’efficience des méthodes de diagnostic au laboratoire d’analyses biomédicales.
Mots-clés : staphylocoagulase libre, plasma de lapin, efficience.


ABSTRACT
Objective: The high cost of lyophilized rabbit’s plasma, which is used for the identification of Staphylococcus aureus, is one of the reasons of inadequacy between income and expenses of biomedical analysis laboratories. To circumvent this difficulty, laboratory technicians resort to less expensive methods of identification. This work was done to determine the lowest volume of rabbit’s plasma that can reveal the presence of free staphylocoagulase.
Methodology and results: To do this, 25 strains of Staphylococcus isolated from different biological samples and identified based on the presence of free staphylocoagulase aureus. These strains were subjected to the research of the free staphylocoagulase with decreasing volumes of 0.4 ml , 0.3 ml , 0.2 ml and 0.1 ml of lyophilized rabbit plasma .All the bacterial strains showed a positive result in 24 hours with 0.2 ml as the smallest volume. The coagulum formed has the same consistency as the one obtained with the indicated volume of 0.5 ml by the normal protocol. In total, the volume of rabbit plasma may therefore be reduced from 0.5 to 0.2 ml without any impact on the quality of the result.
Conclusion and application of results: Thus, the volume of rabbit plasma can be reducing from 0.5 to 0.2 ml without any impact on the quality of the result. This is very interesting for the African laboratories which could improve the efficiency of techniques.
Keywords: Free staphylocoagulase, rabbit plasma, efficiency.


FULL PAPER [PDF AVAILABLE HERE]

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