Journal of Animal and Plant Sciences
J. Anim. Plant Sci. [ISSN 2071 - 7024]
Volume 22(2): 3419 - 3432. Published July, 2014.
Purification and Characterization of Sweet Potato (Ipomoea Batatas) Peroxidase
Mamounata Diao1*, Brice N’cho Ayékoué2, Crépin I. Dibala1, Soumaïla Dabonné2 and Mamoudou H. Dicko1.
1Laboratoire de Biochimie Alimentaire, Enzymologie, Biotechnologie
Industrielle et Bioinformatique (BAEBIB), UFR-SVT, Université de
Ouagadougou, Burkina Faso. 09 BP 848 Ouagadougou 09 (Burkina Faso).
2Laboratoire de biocatalyse et de bioprocédés. Université Nangui Abrogoua, 02 BP 801 Abidjan 02 Côte d’Ivoire.
*Correspondance: Mamounata DIAO; PhD. Phone: (+226) 78620628 /76531787;
Fax: (+226) 50307242; Email: mamounadiao@yahoo.fr; mdicko@univ-ouaga.bf; mamoudou_dicko2004@yahoo.fr
Keywords: peroxidase, characterization, Sweet potato, phenolic compound.
1 ABSTRACT
Peroxidase (POX), a biotechnologically important enzyme was purified
from bulb of Ipomoea batatas and characterized for use in the
bioconversion of phenolic compounds from industrial wastewater.
Purified peroxidase (POXp) was obtained using the gel-filtration
chromatography and anion exchange chromatography. A final yield of 18 %
was obtained with 20.5 as purification fold. Electrophoresis on
SDS-PAGE and native-PAGE showed that POXp is monomeric with a molecular
weight of 44 kDa. The optimum pH and optimum temperature were
respectively 6.0 and 45 ° C. All enzyme activity was retained for 2
hours at pH values between 4.0 to 9.0. While the enzyme was partially
inhibited by SDS, PCMB and sodium disulfide, it was completely
inhibited by ascorbic acid, citric acid, sodium azide and sodium
thiosulfate. The presence of Calcium (Ca2+), Copper (Cu2+), Barium
(Ba2+), Manganese (Mn2+) and polyethylene glycol increased POXp
activity but it was reduced by Magnesium (Mg2+), Sodium (Na+),
Potassium (K+) and EDTA. However, Zinc (Zn2+) had no effect on POXp.
The enzyme oxidized a wide range of phenolic substrates, the greatest
rate of oxidation being obtained with guaiacol. These properties may
allow the use of these enzymes for bioremediation of industrial
wastewater containing phenolic compounds.
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